Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: IL-7Rα and L-selectin, but not CD103 or CD34, are required for murine peanut-induced anaphylaxis

doi: 10.1186/1710-1492-8-15

Figure Lengend Snippet: IL-7Rα and L-selectin, are required for murine peanut-induced anaphylaxis. Mice were initially sensitized using peanut antigen and cholera toxin via oral gavage for 4 consecutive weeks. Two weeks following the final sensitization, mice were challenged i.p. with crude peanut extract. Body temperature ( A , D ) and average observed clinical scores ( B , E ) monitored every 10 minutes for 40 minutes post-injection. Following the 40-minute endpoint, blood levels of histamine were assayed ( C , F ). Control mice were challenged with peanut immediately before monitoring. ( IL7R −/− and Ly5.1 n = 3, representative of 4 experiments; L-Sel −/− n = 8, Bl/6 n = 7, and control mice n = 3, representative of 5 experiments; *represents p < 0.05; **represents p < 0.01; Error bars = SEM).

Article Snippet: C57BL/6, CD103 ( Cd103 −/− ) and IL-7 receptor ( IL7R −/− ) deficient mice were purchased from The Jackson Laboratory.

Techniques: Injection, Control

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: IL-7Rα and L-selectin, but not CD103 or CD34, are required for murine peanut-induced anaphylaxis

doi: 10.1186/1710-1492-8-15

Figure Lengend Snippet: Total and antigen-specific IgE in plasma of IL7R −/− and L-sel −/− mice. Following peanut sensitization and challenge, blood levels of total IgE ( A , B ) and IgE-mediated CPE binding ( C , D ) were assayed at the 40 minute endpoint. ( IL7R −/− n = 3 and Ly5.1 n = 4, representative of 3 experiments; L-Sel −/− n = 5 and Bl/6 n = 7, representative of 4 experiments; ***represents p < 0.001; Error bars = SEM).

Article Snippet: C57BL/6, CD103 ( Cd103 −/− ) and IL-7 receptor ( IL7R −/− ) deficient mice were purchased from The Jackson Laboratory.

Techniques: Clinical Proteomics, Binding Assay

Journal: Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology

Article Title: IL-7Rα and L-selectin, but not CD103 or CD34, are required for murine peanut-induced anaphylaxis

doi: 10.1186/1710-1492-8-15

Figure Lengend Snippet: Equivalent passive systemic anaphylaxis induction in L-sel −/− and IL7R −/− mice. Mice were initially injected i.v. with anti-DNP IgE to simulate sensitization. The next day, mice were injected i.v. with DNP-HSA to induce passive systemic anaphylaxis. Body temperature measurements in L-sel −/− ( A ) and IL7R −/− ( D ) mice were taken at 5-minute intervals for 60 minutes post-injection. We identified maximal individual temperature decreases ( B , E ) and assessed serum histamine levels ( C , F ) in animals sacrificed 5 minutes post-injection. (n = 4-5, *represents p < 0.05; **represents p < 0.01; Error bars = SEM)

Article Snippet: C57BL/6, CD103 ( Cd103 −/− ) and IL-7 receptor ( IL7R −/− ) deficient mice were purchased from The Jackson Laboratory.

Techniques: Injection

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: (A) Plasma IL-7 concentrations in IL-7-treated and untreated macaques. IL-7-treated animals received 7 weekly injections of 50 µg/kg of body weight of a recombinant, fully glycosylated form of simian IL-7 (the grey shaded area indicates the IL-7-treatment period). The asterisks denote significant differences with baseline values (p<0.05 by paired Student's t test). (B) CD127 expression on total, naïve, memory and effector CD3 + T cells from untreated (blue) and IL-7-treated (red) animals. Average values of mean fluorescence intensity (MFI) ± standard error of the mean (SEM) from each group of macaques are shown. Blue and red asterisks denote significant differences with baseline values (day −7) in untreated and treated animals, respectively (* p<0.05, ** p<0.01, by paired Student's t test).

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Recombinant, Expressing, Fluorescence

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: Mean levels (± SEM) of SIV plasma viremia (A) and p27 Gag antigenemia (B) in untreated (blue) and IL-7-treated (red) animals. No significant differences were observed between the two groups of animals, with the only exception of SIV plasma viremia on day 4 post-infection, when IL-7-treated animals showed higher levels compared to untreated controls (indicated by the asterisk; p<0.05, by Wilcoxon rank sum test). The grey-shaded area indicates the IL-7-treatment period. (C) Mean number of genome equivalent copies (± SEM) of SIV proviral DNA in mononuclear cells from peripheral blood (days 14 and 77 post-infection) and lymphoid tissues (GALT, days 14–16; axillary lymph nodes, days 25–27 post-infection). No significant differences were observed between the two groups of animals, p>0.05 by Wilcoxon rank sum test.

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Infection

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: (A) Mean absolute numbers (± SEM) of circulating total, naïve, memory and effector CD4 + and CD8 + T cells in untreated (blue) and IL-7-treated (red) animals. The naïve (CD28 + 95 − ), memory (CD28 + 95 + ) and effector (CD28 − 95 + ) T-cell subsets were identified using a combination of mAbs against CD28 and CD95. (B) Subset analysis of memory CD4 + and CD8 + T cells in untreated (blue) and IL-7-treated (red) animals. The various memory T-cell subsets (central memory, CM; transitional memory, TM; and effector memory, EM) were identified using a combination of CD28, CD95, CD62L and CD197/CCR7. Absolute counts for each T-lymphocyte subpopulation were calculated by multiplying the percent values obtained by flow cytometry by the total lymphocyte counts obtained from the complete blood counts (CBC). The grey shaded area indicates the IL-7-treatment period; blue and red asterisks denote significant differences versus baseline values in untreated and IL-7-treated animals, respectively (* p<0.05, ** p<0.01, by paired Student's t test).

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Flow Cytometry

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: (A) Mean levels (± SEM) of cellular proliferation, as measured by expression of the cell-cycling marker Ki67, in CD4 + and CD8 + T cells freshly isolated from untreated (blue) and IL-7-treated (red) animals. (B) Mean levels (± SEM) of spontaneous apoptosis, as measured by Annexin-V binding, in circulating CD4 + and CD8 + T cells from untreated (blue) and IL-7-treated (red) animals. (C) Average MFI levels (± SEM) of Bcl-2 expression in circulating CD4 + and CD8 + T cells from untreated (blue) and IL-7-treated (red) animals. The grey shaded area indicates the IL-7-treatment period. The asterisks denote significant differences between untreated and IL-7-treated animals (* p<0.05, ** p<0.01, by Wilcoxon rank sum test).

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Expressing, Marker, Isolation, Binding Assay

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: Lymph node (upper panels) and gut (lower panels) biopsies were obtained from all animals on days 25–27 and on days 14–16 post-infection, respectively. (A) Mean levels of spontaneous apoptosis, as measured by Annexin-V binding, in total, naïve, memory and effector CD4 + and CD8 + T cells isolated from axillary lymph node and terminal ileum biopsies from untreated (blue) and IL-7-treated (red) animals. The differences between untreated and IL-7-treated animals were analyzed by unpaired t-test (similar results were obtained by Wilcoxon rank sum test). (B) Mean levels of spontaneous apoptosis, as measured by Annexin-V binding, on total, naïve, memory and effector CD4 + and CD8 + T cells freshly isolated from axillary lymph node and terminal ileum biopsies from untreated (blue) and IL-7-treated (red) animals, after the exclusion of 4 rapid progressor (RP) animals. The differences between untreated and IL-7-treated animals were analyzed by unpaired t-test.

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Infection, Binding Assay, Isolation

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: Mean absolute numbers (± SEM) of total CD4 + and CD8 + T cells responding to overlapping peptides derived from the Tat and Gag proteins of SIV in untreated (blue) and IL-7-treated (red) animals. The asterisk indicates a significant difference between total responses in IL-7-treated vs. untreated macaques.

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Derivative Assay

Journal: PLoS Pathogens

Article Title: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques

doi: 10.1371/journal.ppat.1002636

Figure Lengend Snippet: (A) Mean absolute numbers (± SEM) of CD4 + and CD8 + T cells producing one cytokine (single-producer, SP), two cytokines (double-producer, DP) or all three cytokines (triple-producer, TP) in response to SIV Tat peptide stimulation in untreated (shades of blue) and IL-7-treated (shades of red) animals. The asterisks indicate significant differences between SP T cells in untreated vs. IL-7-treated animals, as analyzed by Wilcoxon rank sum test. (B) Mean absolute numbers of SIV Tat-responding CD4 + and CD8 + T cells in untreated (U) and IL-7-treated (IL-7) animals. The bars indicate the mean numbers of total responding cells; the colors indicate the mean numbers of IFN-γ, IL-2, or MIP-1β SP cells (shades of blue), IFN-γ/IL-2, IFN-γ/MIP-1β or IL-2/MIP-1β DP cells (shades of green), and IFN-γ/IL-2/MIP-1β TP cells (purple). The numbers above each bar indicate the fraction of monkeys that gave a measurable response over background to SIV Tat peptides at the corresponding time point. The grey shaded areas indicate the IL-7-treatment period. The asterisk indicates a significant difference between total responses in IL-7-treated vs, untreated macaques. (C) Qualitative analysis of SIV Tat-specific CD8 + T-cell responses in IL-7-treated and untreated macaques on day 62 post-infection. The pie charts indicate the average contribution of the various functional subpopulations of Tat-responding CD8 + T cells (single-, double- and triple-producing, SP, DP, TP, respectively) to the total number of Tat-responding cells in untreated and IL-7-treated animals at day 62 post-infection. The p value was calculated by permutation analysis using the Spice software.

Article Snippet: Additional mAbs were used to measure the expression of other cell-surface markers, including the IL-7 receptor-α/CD127-PE (clone hIL-7R-M21), CCR5-PE (clone 3A9), CXCR4-PE (clone 12G5) and the activation markers HLA-DR-PE (clone L243/G46-6) and CD25-PE (clone M-A251) (all from BD Biosciences).

Techniques: Infection, Functional Assay, Software